LOCUS pcDNA3.1-TFRC_EC 7456 bp DNA circular SYN 09-OCT-2026 DEFINITION GeneLoop design example with a later synonymous correction. Not the original run's verified revision; experimental function is unproven. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT GeneLoop dev snapshot Timestamp: 2026-10-09T13:10:43.816Z Snapshot: Corrected GeneLoop example FEATURES Location/Qualifiers source 1..7456 /mol_type="other DNA" enhancer 235..614 /label="CMV enhancer" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" promoter 615..818 /label="CMV promoter" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" promoter 863..881 /label="T7 promoter" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" misc_feature 907..3009 /label="IgK-TFRC_ECD-His6_opt" /ApEinfo_fwdcolor="#c026d3" /ApEinfo_revcolor="#c026d3" polyA_signal 3053..3277 /label="bGH poly(A) signal" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" rep_origin 3323..3751 /label="f1 ori" /ApEinfo_fwdcolor="#10b981" /ApEinfo_revcolor="#10b981" promoter 3765..4094 /label="SV40 promoter" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" rep_origin 3945..4080 /label="SV40 ori" /ApEinfo_fwdcolor="#10b981" /ApEinfo_revcolor="#10b981" CDS 4161..4955 /label="NeoR/KanR" /ApEinfo_fwdcolor="#4ade80" /ApEinfo_revcolor="#4ade80" polyA_signal 5129..5250 /label="SV40 poly(A) signal" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" protein_bind 5323..5339 /label="lac operator" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" promoter complement(5347..5377) /label="lac promoter" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" protein_bind 5392..5413 /label="CAP binding site" /ApEinfo_fwdcolor="#00e5ff" /ApEinfo_revcolor="#00e5ff" rep_origin complement(5701..6289) /label="ori" /ApEinfo_fwdcolor="#10b981" /ApEinfo_revcolor="#10b981" CDS complement(6460..7320) /label="AmpR" /ApEinfo_fwdcolor="#4ade80" /ApEinfo_revcolor="#4ade80" promoter complement(7321..7425) /label="AmpR promoter" /ApEinfo_fwdcolor="#4ade80" /ApEinfo_revcolor="#4ade80" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gccaccatgg acatgagggt gcctgcccaa cttcttggcc tgctgcttct gtggcttcct 961 ggcgctaagt gctgcaaggg cgtggagccc aagacagaat gtgagaggct ggccggaaca 1021 gagagccctg ttagggagga gcctggcgaa gacttccctg ctgccaggag actgtactgg 1081 gacgacctga agagaaagct gagcgagaag ctggacagca ccgacttcac cggcaccatc 1141 aagctgctga acgagaactc ttacgtgcct agggaggccg gcagccagaa ggacgagaac 1201 cttgccctgt atgtggagaa ccagttcagg gagttcaagc tgagcaaggt gtggagggac 1261 cagcacttcg tgaagatcca ggtgaaggac agcgcccaga acagcgtgat catcgtggac 1321 aagaacggca ggctggtgta cctggtggag aaccctggcg gctacgtggc ttacagcaaa 1381 gctgccaccg tgaccggaaa gctggtgcac gccaacttcg gcaccaagaa ggacttcgag 1441 gacctgtaca cccccgtgaa cggcagcatc gtgatcgtga gggctggcaa gatcaccttc 1501 gccgagaagg tggccaacgc cgaaagcctg aacgccatcg gagtgctgat ctacatggac 1561 cagaccaagt tccccatcgt gaacgccgag ctgagcttct tcggacacgc ccatctggga 1621 acaggcgatc cctatacccc cggcttccct agcttcaacc acacccagtt ccccccctct 1681 agaagcagcg gcctgcccaa tattcccgtg cagaccatct ctagggccgc tgctgagaag 1741 ctgttcggca acatggaggg cgactgtcct agcgactgga agaccgactc tacctgcagg 1801 atggtgacca gcgagagcaa gaacgtgaag ctgaccgtga gcaacgtgct gaaggagatc 1861 aagatcctga acatcttcgg cgtgatcaag ggcttcgtgg agcccgacca ctacgttgtt 1921 gtgggcgctc aaagggatgc ttggggacct ggagctgcta agtctggagt gggaaccgcc 1981 ctgctgctga agctggccca gatgttcagc gacatggtgc tgaaggacgg cttccagccc 2041 agcaggagca tcatcttcgc cagctggagc gctggagact tcggatctgt gggcgctacc 2101 gaatggctgg aaggctacct gagcagcctg cacctgaagg ccttcaccta catcaacctg 2161 gacaaggccg tgttgggcac aagcaacttc aaggtgagcg ccagccccct gctgtacacc 2221 ctgatcgaga agaccatgca gaacgtgaag caccccgtga ccggccagtt cctgtaccag 2281 gacagcaact gggcctctaa ggtggagaag ctgaccctgg acaacgccgc cttccccttc 2341 ctggcctatt ctggcattcc tgccgtgagc ttctgcttct gcgaggacac cgactacccc 2401 tacctgggca ccaccatgga cacctacaag gagctgatcg agaggatccc cgagctgaac 2461 aaagtggcta gggccgctgc cgaagttgcc ggacagttcg tgatcaagct gacccacgac 2521 gtggagctga acctggacta cgagaggtac aacagccagc tgctgagctt cgtgagggac 2581 ctgaaccagt acagggccga catcaaggag atgggcctga gcctgcagtg gctgtacagc 2641 gctagaggcg acttcttcag agccaccagc agactgacca ccgacttcgg caacgccgag 2701 aagacagaca ggttcgtgat gaagaagctg aacgacaggg tgatgagggt ggagtaccac 2761 ttcctgagcc cctacgtgag ccccaaagag agccccttca ggcatgtgtt ttggggctct 2821 ggaagccaca ccctgcccgc tctgctggag aaccttaagc tgaggaagca gaacaacggc 2881 gccttcaacg agaccctgtt caggaaccag ctggccttag ctacctggac cattcagggc 2941 gccgctaatg ccctgtctgg cgacgtgtgg gatatcgaca acgagttcca ccaccaccac 3001 caccactgac tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct 3061 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 3121 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 3181 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac 3241 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc 3301 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 3361 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 3421 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 3481 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 3541 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 3601 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 3661 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 3721 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt 3781 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 3841 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 3901 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 3961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 4021 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 4081 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga 4141 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 4201 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 4261 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 4321 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg 4381 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 4441 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 4501 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 4561 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 4621 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 4681 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 4741 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 4801 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 4861 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 4921 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 4981 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 5041 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 5101 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa 5161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 5221 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta 5281 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 5341 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 5401 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 5461 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 5521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 5701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 5761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 5821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 5881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 5941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6121 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 6181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6421 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6481 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6541 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6601 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6661 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 6721 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 6781 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 6841 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 6901 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 6961 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7021 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7081 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7141 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7201 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7261 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7321 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7381 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7441 agtgccacct gacgtc //